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Keisuke Yamada

About Project

Personality of Slime Molds

Description

As we, humans, are quite different with each other’s, slime molds are also different among media. I tried to investigate if slime molds have individual personalities.

Wet Experiment

Dry Simulation

DIY Synbio

• Description

  Using DIY bio techniques, I would build a biomolecular circuit that demonstrates turing model.

• Midterm Presentation

  video

• Plan

  3/10(Sat) project planning

  3/13(Tue) making Calcium competent cells(3days to prepare)

  - E.coli
  - CaCl2
  - MgCl2
  - glycerol
  - incubator 37℃
  - icebox filled with ice
  - OD meter
  - freezer
  - and...
  

  (3/17(Sat) absent)

  3/20(Tue) transformation experiment

  - competent cells
  - DNA to transform(plasmid with antibiotic resistance and GFP gene)
  - media including antibiotic
  - microfuge tubes
  - incubator 37℃
  - waterbath 42℃
  - icebox filled with ice
  - and...
  

  3/24(Sat)

  3/27(Tue)

  3/31(Sat)

• Link

  about Turing model in E.coli

  https://www.brh.co.jp/seimeishi/journal/011/to_1.html

  https://blogs.yahoo.co.jp/crazy_tombo/47904461.html

  https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-09J09649/

  making competent cells

  http://mcb.berkeley.edu/labs/krantz/protocols/calcium_comp_cells.pdf

  https://openwetware.org/wiki/Preparing_chemically_competent_cells

  http://blog.addgene.org/save-time-and-money-by-making-your-own-competent-cells

  transformation

  https://openwetware.org/wiki/Transforming_chemically_competent_cells

  https://www.addgene.org/protocols/bacterial-transformation/